Introduction to Mammalian Bioprocessing

This short course will give you a hands-on introduction to the production of biopharmaceutical proteins in mammalian cells. Through a mix of hands-on lab, theoretical exercises and lectures, the course will guide you through topics on mammalian cell cultivation, bioprocess modes, bioprocess control, cell line engineering, single-use equipment, and with a particular focus on perfusion bioprocess. Upon completion of the course, you will have sufficient overview, theoretical knowledge, and practical hands-on experience to e.g., get more company-specific training; better understand and support the upstream department; or participate in management level decision-making on the covered topics. In addition to extensive hands-on experience with the process, this course will also address many of the common concerns when implementing single-use processes – i.e., sterile connections, environmental concerns/carbon footprint, business economics, and validation.

At the end of this course, participants should be able to: • Design of animal cell culture bioreactors, including suspension and immobilized cell bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors • Identify advantages and disadvantages of single-use technologies relative to multi-use equipment • Understand the pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion) • Setup and operation of single-use options for upstream manufacturing (rocker, stirred tank and perfusion) • Advanced automation and control of animal cell culture processes, including bioreactor Process Analytical Technology (PAT) • Basic cost calculations to compare single-use options to more traditional multi-use set ups, to provide justification for single-use technologies.

Nongraded

This course is intended for participants who are: • Professionals within the biopharmaceutical industry who want to gain hands-on experience with mammalian bioprocessing equipment • Professionals who want to gain an understanding of upstream mammalian bioprocessing • Professionals working indirectly with mammalian bioprocessing who want to better understand it Prerequisites Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences.